Biological properties of Ceraputty as a retrograde flling material: an in vitro study on hPDLSCs
Author/s
López García, Sergio; Sanz, José Luis; Forner, Leopoldo; Lozano, Adrián; Murcia, Laura; [et al.]Date
2023-08-01Discipline/s
OdontologíaSubject/s
BioactivityBiocompatibility
Biodentine
Ceraputty
Endodontics
Endosequence root repair material
Periodontal ligament stem cells
Abstract
Objectives. To assess the cytocompatibility and bioactive potential of the new calcium silicate-based cement Ceraputty on human periodontal ligament stem cells (hPDLSCs) compared to Biodentine and Endosequence BC root repair material (ERRM).
Materials and methods. hPDLSCs were isolated from extracted third molars from healthy donors. Standardized sample discs and 1:1, 1:2, and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, osteo/cemento/odontogenic marker expression via RT-qPCR, and cell calcifed nodule formation via Alizarin Red S staining. hPDLSCs cultured in unconditioned or osteogenic media were used as negative and positive control groups, respectively. Statistical analysis was performed using one-way ANOVA or two-way ANOVA and Tukey’s post hoc test. Statistical signifcance was established at p<0.05.
Results. The highest Ca2+ ...