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dc.contributor.authorWu, Jun
dc.contributor.authorPlatero Luengo, Aida
dc.contributor.authorSakurai, Masahiro
dc.contributor.authorSugawara, Atsushi
dc.contributor.authorGil, Maria Antonia
dc.contributor.authorYamauchi, Takayoshi
dc.contributor.authorSuzuki, Keiichiro
dc.contributor.authorBogliotti, Yanina Soledad
dc.contributor.authorMorales Valencia, Mariana
dc.contributor.authorOkumura, Daiji
dc.contributor.authorLuo, Jingping
dc.contributor.authorVilariño, Marcela
dc.contributor.authorParrilla, Inmaculada
dc.contributor.authorSoto, Delia Alba
dc.contributor.authorCristina A, Martinez
dc.contributor.authorHishida, Tomoaki
dc.contributor.authorSánche Bautista, Sonia
dc.contributor.authorWang, Huili
dc.contributor.authorNohalez, Alicia
dc.contributor.authorAizawa, Emi
dc.contributor.authorMartinez Redondo, Paloma
dc.contributor.authorOcampo, Alejandro
dc.contributor.authorReddy, Pradeep
dc.contributor.authorRoca, Jordi
dc.contributor.authorMaga, Elizabeth A
dc.contributor.authorConcepcion, Rodriguez Esteban
dc.contributor.authorBerggren, W Travis
dc.contributor.authorNuñez Delicado, Estrella
dc.contributor.authorLajara Blesa, Jerónimo
dc.contributor.authorGuillén, Isabel
dc.contributor.authorGuillen, Pedro
dc.contributor.authorCampistol, Josep M
dc.contributor.authorMartinez, Emilio A
dc.contributor.authorRoss, Pablo Juan
dc.contributor.authorIzpisua Belmonte, Juan Carlos
dc.contributor.authorMartinez Martinez, Llanos
dc.date.accessioned2024-07-22T09:13:31Z
dc.date.available2024-07-22T09:13:31Z
dc.date.issued2017-01-26
dc.identifier.isbn0092-8674
dc.identifier.urihttp://hdl.handle.net/10952/8055
dc.description.abstractInterspecies blastocyst complementation enables organ-specific enrichment of xenogenic pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice. Besides gaining insights into species evolution, embryogenesis, and human disease, interspecies blastocyst complementation might allow human organ generation in animals whose organ size, anatomy, and physiology are closer to humans. To date, however, whether human PSCs (hPSCs) can contribute to chimera formation in non-rodent species remains unknown. We systematically evaluate the chimeric competency of several types of hPSCs using a more diversified clade of mammals, the ungulates. We find that naïve hPSCs robustly engraft in both pig and cattle pre-implantation blastocysts but show limited contribution to post-implantation pig embryos. Instead, an intermediate hPSC type exhibits higher degree of chimerism and is able to generate differentiated progenies in post-implantation pig embryos.es
dc.language.isoenes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCRISPR-Cas9es
dc.subjectPluripotent stem cell (PSC)es
dc.subjectOrgan and tissue generationes
dc.subjectHuman naïve pluripotent stem cellses
dc.subjectBlastocyst complementationes
dc.titleInterspecies Chimerism with Mammalian Pluripotent Stem Cellses
dc.typearticlees
dc.rights.accessRightsopenAccesses
dc.journal.titleCelles
dc.volume.number168es
dc.issue.number3es
dc.description.disciplineMedicinaes
dc.identifier.doi10.1016/j.cell.2016.12.036es


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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