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One-Electron Reduction of 6-Hydroxydopamine Quinone is Essential in 6-Hydroxydopamine Neurotoxicity
dc.contributor.author | Villa, Mónica | |
dc.contributor.author | Muñoz, Patricia | |
dc.contributor.author | Ahumada Castro, Ulises | |
dc.contributor.author | Paris, Irmgard | |
dc.contributor.author | Jiménez, Ana | |
dc.contributor.author | Martínez Alcalá, Isabel | |
dc.contributor.author | Sevilla, Francisca | |
dc.contributor.author | Segura Aguilar, Juan | |
dc.date.accessioned | 2024-07-10T10:59:41Z | |
dc.date.available | 2024-07-10T10:59:41Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1476-3524 | |
dc.identifier.uri | http://hdl.handle.net/10952/7964 | |
dc.description.abstract | 6-Hydroxydamine has widely been used as neurotoxin in preclinical studies related on the neurodegenerative process of dopaminergic neurons in Parkinson’s disease based on its ability to be neurotoxic as a consequence of free radical formation during its auto-oxidation to topaminequinone. We report that 50-µM 6-hydroxydopamine is not neurotoxic in RCSN-3 cells derived from substantia nigra incubated during 24 h contrasting with a significant sixfold increase in cell death (16 ± 2 %; P < 0.001) was observed in RCSN-3NQ7 cells expressing a siRNA against DT-diaphorase that silence the enzyme expression. To observe a significant cell death in RCSN-3 cells induced by 6-hydroxydopamine (24 ± 1 %; P < 0.01), we have to increase the concentration to 250 μm while a 45 ± 2 % cell death (P < 0.001) was observed at this concentration in RCSN-3NQ7 cells. The cell death induced by 6-hydroxydopamine in RCSN-3NQ7 cells was accompanied with a (i) significant increase in oxygen consumption (P < 0.01), (ii) depletion of reduced glutathione and (iii) a significant decrease in ATP level (P < 0.05) in comparison with RCSN-3 cells. In conclusion, our results suggest that one-electron reduction of 6-hydroxydopamine quinone seems to be the main reaction responsible for 6-hydroxydopamine neurotoxic effects in dopaminergic neurons and DT-diaphorase seems to play an important neuroprotective role by preventing one-electron reduction of topaminequinone. | es |
dc.language.iso | en | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | 6-Hydroxydopamine | es |
dc.subject | Quinone metabolism | es |
dc.subject | Dopamine | es |
dc.subject | Oxidation | es |
dc.subject | Quinone | es |
dc.subject | Parkinson’s disease | es |
dc.subject | Glutathione | es |
dc.title | One-Electron Reduction of 6-Hydroxydopamine Quinone is Essential in 6-Hydroxydopamine Neurotoxicity | es |
dc.type | article | es |
dc.rights.accessRights | openAccess | es |
dc.journal.title | Neurotoxicity Research | es |
dc.description.discipline | Ciencias Ambientales | es |
dc.identifier.doi | 10.1007/s12640-013-9382-7 | es |