Carvacrol and HP-β-Cyclodextrin Complexes: Extensive Characterization and Potential Cytotoxic Effect in Human Colorectal Carcinoma Cells
Author/s
Rodríguez López, María Isabel; Mercader Ros, María Teresa; Pérez Garrido, Alfonso; Pérez Sánchez, Horacio; Pellicer, José Antonio; [et al.]Date
2022-11-29Discipline/s
FarmaciaMedicina
Subject/s
HP-β-cyclodextrinsChemical characterization
Cell viability
Solid complexes
Carvacrol
Microwave irradiation
Abstract
The aim of this study was to obtain solid carvacrol-cyclodextrin (CD) complexes for use in the pharmaceutical industry. To this end, the complexation of carvacrol at different pH values was studied in detail, to determine the type of CD and the reaction environment that supported the highest amount of encapsulated carvacrol. Evidence of the capability of hydroxypropyl-β-cyclodextrins (HP-β-CD) to form inclusion complexes with carvacrol (KC = 5042 ± 176 L mol−1) and more high complexation efficiency (2.824) was demonstrated for HP-β-CDs using two different energy sources, ultrasound (US) (KC = 8129 ± 194 L mol−1 24 h) and microwave irradiation (MWI) (KC = 6909 ± 161 L mol−1), followed by spraying the resulting solution in a spray dryer. To confirm complex formation, the complexes were characterized using various instrumental methods to corroborate the carvacrol incorporation into the hydrophobic cavity of HP-β-CD. The obtained carvacrol solid complexes were analyzed by 1H nuclear magnet...