Antimicrobial properties of different multilayered scaffolds complexed with metronidazole and metronidazole-ozone

Abstract

This study compares two different scaffolds (dicalcium silicate – Ca2SiO4 – core covered with a glass of composition Ca2P6O17 doped with lithium and with strontium) loaded with metronidazole alone and with metronidazole combined with ozone. Complexes metronidazole-2-hydroxypropyl-β-cyclodextrins (HP-β-CDs) were used to increase the solubility and availability of metronidazole. The concentration of metronidazole used was 40.8mg/mL (58.4mM), with a molar ratio of 1:1 and 1.5mL of ozone oil were added to the different scaffolds. The disk diffusion method against the Gram-positive bacterium Staphylococcus aureus and the Gram-negative bacterium Escherichia coli showed microbial inhibition but not statistically significant (p>0.05) halo inhibition produced by metronidazole alone and that produced by metronidazole combined with ozone.

This research is expected to have strong social impact directly affecting the quality of life of those affected by osteonecrosis as these nanostructures anchored to structures with a porous 3D-core could facilitate bone regeneration promoted by cell adhesion, vascularization and nutrients supply.